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The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

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The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii.

Funding for sustainable Open Access incl. Close Go to Workspace. Please provide a name for this query: The factorial experiment consisted of two factors that was arranged by completely randomized design CRD with ten replications. Agriculture General Country of publisher: On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks incubation.


For soybean leaf, the medium was enriched with driselase and pectolyase Y Indonesian, English Full-text formats available: Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau.

Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated in lowlight intensity by constant shaking at rpm in reciprocal water shaker at 30 celcius centigrade. Journal on Agriculture Science.

Looks like Javascript is disabled on your browser. Our members Our publisher members Our sponsors Our volunteers. Protoplast were cultured on the medium of Ikeda and Uchimia.

Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. Indonesia Language of fulltext: Journal Homepage Journal Title: The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours.

Leaf protoplast were releasaed in h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation. Cell wall islasi was observed. Publication Year Publication Year.

The results showed that there was effect of treatment for all variables observed.

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Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and izolasi protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 and between cowpea C4 and cowpea E1. They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker.


The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0. Protoplast were collected by centrifugation of g and a thin layer proto;las the suspension was irradiated with ultraviolet light. Javascript must be enabled for narrowing. Reference Number Reference Number. The first factor was concentrations of sucrose which consists of three levels namely 0.

Isolasi Protoplas secara Enzimatis pada Tanaman Kecipir | Riyadi | Buletin Plasma Nutfah

Isolation of protoplast from soybean, cowpea, and tobacco and their fusion. Fusion was induced with PEG solution according to Uchimia and fused protoplasts were collected by centrifugation of g. Get permanent URL for this record.

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